Inherited hemoglobin disorders are common worldwide due to population migration. These disorders can be characterised by mutations in the globin gene which may result in a structural abnormality or a reduction in the rate of synthesis of one of the globin chains. A structural change may be harmless, however, some may impair function of hemoglobin and oxygen transport. Here, we will describe a SONAR rapid quadrupole scanning data independent acquisition (DIA) method for the precise identification of several variants that may be encountered in either the α- or β-hemoglobin chains.