Poster Presentation 23rd Annual Lorne Proteomics Symposium 2018

A Novel Data Independent Acquisition Method for Hemoglobin Variant Identification in Clinical Research (#85)

Jonathan P Williams 1 , ChristopherChristopher Hughes 1 , Heather A Brown 1 , Keith Richardson 1 , Johanness P.C. Vissers 1 , LeRoy B Martin 1 , Jose Castro-Perez 1
  1. Waters Corp., Milford, MA, United States

Inherited hemoglobin disorders are common worldwide due to population migration. These disorders can be characterised by mutations in the globin gene which may result in a structural abnormality or a reduction in the rate of synthesis of one of the globin chains. A structural change may be harmless, however, some may impair function of hemoglobin and oxygen transport. Here, we will describe a SONAR rapid quadrupole scanning data independent acquisition (DIA) method for the precise identification of several variants that may be encountered in either the α- or β-hemoglobin chains.