Poster Presentation 23rd Annual Lorne Proteomics Symposium 2018

Characterisation of Biotherapeutics Using Native Ion Mobility Mass Spectrometry (#104)

Christopher Buck 1 , Dale A Cooper-Shepherd 2 , Jakub Ujma 2 , Kevin Giles 2 , Nick Tomczyk 2 , Laetitia Denbigh 2
  1. Waters Australia, Burnside, VIC, Australia
  2. Waters Corporation, Manchester, UK

Biopharmaceutical products present a complex analytical challenge to the modern pharma industry. Not only must laboratories characterize the primary structure of protein species, but also the tertiary and quarternary (higher order) structure. One method often used in research laboratories for higher order structure analysis is native ion mobility spectrometry-mass spectrometry (IMS-MS). Due to the expansion of the biopharmaceutical industry and the broadening range of drugs being investigated, instrumentation is required to carry out advanced experiments in a routine manner. We describe a method to carry out native IMS-MS on a routine high resolution LC-IMS-MS platform. We also describe investigations into the applicability of high resolution-IMS-MS (HR-IMS-MS) for biopharmaceutical analysis using a cyclic IMS-enabled research platform.

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