Glycomics is a rapidly emerging field that can be viewed as a complement to other „omics“ approaches. Hence, there is a dramatic increase in the demand for analytical tools and specific databases in glycobiology, respectively, glycobiotechnology. In order to enhance and improve the comparatively small existing glycoanalytical toolbox , automated, sensitive, reliable, high-throughput and high-resolution analysis methods including automated data evaluation are required.
Our approach, is the implementation of cutting edge technologies, like multiplexed capillary gelelectrophoresis with laser induced fluorescence detection (xCGE-LIF) and LC-MS in combination with the development of dedicated software (glyXtoolCE and glyXtoolMS) to further improve their usability and performance. The development of this high-performance glycoanalysis systems (methods, software and database) and their application to different fields with respect to sample preparation, analysis and data analysis is presented [2-6]. Further, their smart applicability is demonstrated for different types of glycosamples, like biopharmaceuticals, vaccines, human stem cells, milk and blood serum [7-11].