Oral Presentation 23rd Annual Lorne Proteomics Symposium 2018

Advances in the Glycoanalytical Toolbox (#25)

Erdmann Rapp 1 2 , Rene Hennig 1 2 , Samantha Cajic 2 , Thilo Muth 1 , Alexander Behne 1 , Markus Pioch 2 , Alexander Pralow 2 , Robert Kottler 2 , Udo Reichl 2 3
  1. glyXera GmbH, Magdeburg, Germany
  2. Max-Planck-Institute for Dynamics of Complex Technical Systems, Magdeburg, Germany
  3. Otto-von-Guericke University, Magdeburg, Germany

Glycomics is a rapidly emerging field that can be viewed as a complement to other „omics“ approaches. Hence, there is a dramatic increase in the demand for analytical tools and specific databases in glycobiology, respectively, glycobiotechnology. In order to enhance and improve the comparatively small existing glycoanalytical toolbox [1], automated, sensitive, reliable, high-throughput and high-resolution analysis methods including automated data evaluation are required.

Our approach, is the implementation of cutting edge technologies, like multiplexed capillary gelelectrophoresis with laser induced fluorescence detection (xCGE-LIF) and LC-MS in combination with the development of dedicated software (glyXtoolCE and glyXtoolMS) to further improve their usability and performance. The development of this high-performance glycoanalysis systems (methods, software and database) and their application to different fields with respect to sample preparation, analysis and data analysis is presented [2-6]. Further, their smart applicability is demonstrated for different types of glycosamples, like biopharmaceuticals, vaccines, human stem cells, milk and blood serum [7-11].