Poster Presentation 23rd Annual Lorne Proteomics Symposium 2018

Novel capillary-flow LC HRAM MS platform for fast targeted analysis and robust profiling of complex samples (#154)

Steve Binos 1 , Alexander Boychenko 2 , Martin Ruehl 2 , Christopher Pynn 2 , Mike Baynham 3 , Wim Decrop 2 , Alexander Harder 4
  1. Thermo Fisher Scientific, Parkville, VIC, Australia
  2. Thermo Fisher Scientific, Germering., Germany
  3. Thermo Fisher Scientific, Runcorn, UK
  4. Thermo Fisher Scientific, Bremen, Germany

Capillary flow LC-MS (capLC-MS) with 100-300 µm inner diameter (ID) columns and flow rates from 1 to 10 µL/min provide increased MS sensitivity, lower solvent consumption, and reduced MS contamination  compared with typical analytical flow LC-MS .Here we present a highly robust novel capillary-flow LC-MS platform that combines the Thermo Scientific™ capillary-flow UltiMate™ 3000 RSLCnano system (capLC), the new 150 µm ID Thermo Scientific™ EASY-Spray™ column and the new Thermo Scientific™ Q Exactive™ HF-X mass spectrometer. Both shotgun and targeted proteomics experiments (Full-scan MS, Data-Dependent Acquisition (DDA), and Parallel-Reaction Monitoring (PRM)) were employed to verify the performance and robustness of the novel capLC-MS platform. We show that the novel capLC-MS platform is a sensitive and reliable solution for shotgun and targeted high-resolution accurate-mass (HRAM) proteomics experiments that can be used for routine proteome profiling of complex samples including bio-fluids as well as targeted high-throughput quantification.