Capillary flow LC-MS (capLC-MS) with 100-300 µm inner diameter (ID) columns and flow rates from 1 to 10 µL/min provide increased MS sensitivity, lower solvent consumption, and reduced MS contamination compared with typical analytical flow LC-MS .Here we present a highly robust novel capillary-flow LC-MS platform that combines the Thermo Scientific™ capillary-flow UltiMate™ 3000 RSLCnano system (capLC), the new 150 µm ID Thermo Scientific™ EASY-Spray™ column and the new Thermo Scientific™ Q Exactive™ HF-X mass spectrometer. Both shotgun and targeted proteomics experiments (Full-scan MS, Data-Dependent Acquisition (DDA), and Parallel-Reaction Monitoring (PRM)) were employed to verify the performance and robustness of the novel capLC-MS platform. We show that the novel capLC-MS platform is a sensitive and reliable solution for shotgun and targeted high-resolution accurate-mass (HRAM) proteomics experiments that can be used for routine proteome profiling of complex samples including bio-fluids as well as targeted high-throughput quantification.