Oral Presentation 23rd Annual Lorne Proteomics Symposium 2018

Application of quantitative proteomics to' leaky-gut' and intestinal barrier dysfunction - shifting landscapes in the understanding of IBD pathophysiology (#59)

Valerie Wasinger 1 2 , Rupert Leong 3 , Yunki Yau 4
  1. School of Medical Sciences, The University of NSW, Sydney, NSW, Australia
  2. Mark Wainwright Analytical Centre, The University of NSW, Sydney, NSW, Australia
  3. Endoscopy, Concord Hospital, Concord, NSW, Australia
  4. Mark Wainwright Analytical Centre, The University of NSW, Kensington, NSW, Australia

The aetiology and cure for inflammatory Bowel Disease (IBD) are uncertain. Measures reflectingto disease activity, permeability, and Inflammatory state are available but are limited in their ability to predict or measure ‘clinical remission’ - a newly accepted gold-standard for treatment of this condition. Scientists and clinicians are embracing the concept of intestinal epithelial barrier integrity and its role in the pathogenesis and natural history of IBD.

Peptide biomarkers from the low-mass-plasma proteomes have been identified as significant players in the diagnosis of IBD, the differentiation of active disease and remission, and remission and healthy individuals. These markers have been quantitated using label-free and absolute MRM techniques. Binding partner studies show a novel relationship to endocytic signaling, lipid metabolism and actin nucleation; and additionally correlate to the ‘tissue integrity’ of leaky-gut’ IBD patients.

Modulated proteins in patients with ongoing intestinal damage may be able to predict for relapse and the need to escalate treatment. Markers which can be translated into treatment management able to measure repair of leak, restitution and epithelial cell healing are being sought to manage IBD.

 

  1. Wasinger VC, Yau Y, Duo X, Zeng M, Campbell B, Shin S, Luber R, Redmond D, Leong RW. 2016. Mol. Cell. Prot. 15,1: 256-265.
  2. Yau, Y.Y., Duo, X., Leong, R,W., Wasinger, V.C. 2015. Mol. Cell. Prot. 14,2:441-454.
  3. Chang, J., Leong, R.W., Wasinger, V., Ip, M., Yang, M., Giang Phan, T. 2017. Gastroenterology. S0016-5085,17:35731.